This session will review the basic principles and guidelines for proper Hematoxylin and Eosin Staining so that you can confidently and quickly get to the bottom of the issue. A brief history of H&E will be covered and a look into what pre-analytical processes could affect the H&E stain.
Although xylene has been a staple ingredient in traditional histology practices for decades, there is no denying that safe and effective solutions exist that eliminate unnecessary exposure for histology professionals. The topic of xylene-free histology processing has been growing, and more and more labs are finding success as they transition away from traditional methods of sample handling. Replacing xylene with isopropyl alcohol during tissue processing saves money, is easy to do, and eliminates hazardous chemical exposure. The results offer comparable and, often, superior tissue sectioning and slide quality. Multiple first-hand experiences and examples will be discussed as well as other solutions to reduce chemical exposure risks for the histology professional. This presentation is intended to offer guidance and be a resource for individuals interested in eliminating xylene from their daily histology processes.
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