This is Part II of a two-part series on the evolution of multiplex methods. Read Part I . Using immunofluorescence has several advantages over brightfield IHC assays. Immunofluorescence assays facilitate the visualization of multiple marker colocalization within the same cellular...
This is Part I of a two-part series on the evolution of multiplex methods. Over the last 5 years or so, there have been some major technological advances in the practice of histotechnology, especially compared to when I started my first histology career 33 years ago. At that time, the most...
Immunohistochemistry (IHC) is a useful tool in clinical diagnosis and used to localize specific antigens in tissue sections with labeled antibodies based on antigen-antibody interactions. Immunohistochemically double labeling is a common method used to evaluate two distinct antigens in a single...
Today, immunohistochemistry (IHC) is an essential and routinely used ancillary tool in anatomic pathology for clinical diagnostics and research laboratories. Over the years there have been great advancements in IHC with cancer research and clinical diagnosis, which has improved accuracy,...
My histology lab onboarded polymerase chain reaction (PCR) for dermatomycosis a couple of years ago. I was charged with figuring out the ins and outs of onboarding this new test while making sure we could pass an audit by CLIA and CAP. Little by little, the path to PCR was painstakingly teased...
We know that Immunohistochemistry is the combination of three distinct fields—histology/pathology, immunology, and histochemistry. With IHC we start with our properly prepared tissue samples (histology/pathology), take advantage of the fact that antibodies bind to specific proteins (immunology),...
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NSH Laboratory Webinar Series Laboratory Webinars are a great, inexpensive way to provide continuing education to a large number of employees. The cost for each session is the same regardless of the number of attendees. Why Should I subscribe? *One, affordable price for your entire lab...
Malignant melanoma (MM) remains the deadliest form of skin cancer. The current advances in patient cancer care and treatment for melanoma patients, which include wide local excision as a day case procedure or surgery which involve overnight stay in the hospital, radiotherapy, and chemotherapy....
You may have heard the term companion diagnostic, but what actually is it, and how do companion diagnostics differ from complimentary diagnostics? A companion diagnostic is a test that is required for a patient to receive a specific drug. The drug says on its label, that the test must be run...