If you’re a histology professional considering advancing your career, the QIHC exam might be a worthwhile step. This qualification, offered by the ASCP, focuses on the evolving field of immunohistochemistry (IHC). The QIHC exam tests a range of topics, from general immunology to laboratory operations, and can help you gain recognition and expertise in this specialized area. In this blog, I’ll share my personal journey with immunohistochemistry, my decision to pursue the QIHC qualification, and the study materials that helped me succeed. Whether you’re contemplating taking the exam or simply looking to expand your knowledge, I hope my experience provides valuable insights and encouragement.
My Interest in Immunohistochemistry
My interest in immunohistochemistry began during my histology program internship at a research lab. While I enjoyed microtomy and other aspects of histology, I realized that I did not want a long-term job where I spent my entire shift at a microtome. My first histology job was in a hospital lab where I was at a microtome for most of my shifts. This experience, however, helped me improve my microtomy skills. After working there for over two years, I decided to join a research lab to enhance my immunohistochemistry skills and knowledge. In this lab, which provided histology services for pre-clinical and discovery phases of research, I optimized various antibodies using different detection methods. I was also introduced to immunofluorescence and multiplexing, which I found fascinating! The veterinary pathologists I worked with shared their knowledge during slide reviews, helping me better understand immunohistochemistry and immunofluorescence. This invaluable experience allowed me to develop a deeper understanding of the techniques and their applications. Additionally, being part of a dynamic research environment fostered my curiosity and encouraged me to stay updated with the latest advancements in IHC/IF.
My Decision to Take the QIHC
After gaining a few years of experience in the research lab, I decided to pursue the QIHC qualification from the ASCP. Despite hearing from some that it was pointless and wouldn't result in a raise, I am glad I pursued it anyway. I wanted to challenge myself to gain a deeper understanding of immunohistochemistry and pass the exam. Although I did not get a raise at that job after passing the QIHC, my current role listed "QIHC preferred" on the job posting. Passing the QIHC not only helped me secure my current position but also serves as evidence of my expertise. The qualification has opened doors for me and given me a significant edge in the competitive job market. It has also boosted my confidence in my professional skills and validated my commitment to continuous learning and professional development.
What is the QIHC Exam?
Immunohistochemistry (IHC) is an important area of histology that continues to develop as new technologies are discovered. Although the ASCP does not offer a certification for IHC, they do provide a qualification known as the QIHC. This is a 90-minute timed exam comprising 50 questions on various topics such as general immunology, detection systems, specimen handling, antigen retrieval, staining, and laboratory operations. You can find a detailed topic outline here. The exam costs $250 but the first place to start is your employer. Ask your employer if they will reimburse you for the exam!
My Study Materials
Working in research greatly helped me pass the exam, as I had numerous opportunities to troubleshoot and optimize antibodies. I searched the Histology Professionals Facebook page and the r/histology subreddit on Reddit to see what others were reading to prepare for the exam. Another great place to ask for advice would be The Block. Many recommended the 5th edition of the Dako Education Guide "Immunohistochemical Staining Methods," which I found very useful. This guide can be found online, and I also bought a hard copy on eBay. Most vendors offer some form of immunohistochemistry guide, so I sought out these resources to learn about different IHC/IF products from various companies. At the time, I purchased NSH’s QIHC study course, now the QIHC Study Tool. Some other ways to prepare for the exam are attending the IHC-related workshops at the 2024 NSH Convention, and I also suggest performing immunohistochemistry by hand, if possible. This practical experience is invaluable. Combining practical lab experience, utilizing various study resources, and seeking advice from online histology communities significantly contributed to my success in obtaining the QIHC qualification.
In Conclusion
While the QIHC did not provide immediate benefits, I would not be where I am today without it. The qualification has given me a strong foundation in immunohistochemistry, which has been instrumental in my career progression. It has also demonstrated my commitment to the field and my willingness to invest in my professional development. For anyone considering the QIHC, I can confidently say that the effort and dedication required are well worth the long-term rewards.
Written by: Sara Sheppard McCracken, MHA, HTL(ASCP)QIHC