Histologists know this proverb by heart: “Tissues that didn’t fix well will not process well, will not section well, and will not stain well.” Everything in histology begins with proper fixation. When fixation is compromised, it creates a cascade effect through every subsequent step — processing, embedding, sectioning, staining, and ultimately interpretation. There’s another universal truth that is harder to digest but just as accurate: “Garbage in, garbage out.” We know this. We live this. And yet, fixation is often the one step we have the least control over.
When the Fixation Didn’t Go Well
Recently, our lab received several large pieces of swine heart ...